Research Articles
HITI Revolution: Mastering Homology-Independent Targeted Integration for Advanced Gene Therapies
This article provides a comprehensive exploration of Homology-Independent Targeted Integration (HITI), a groundbreaking CRISPR/Cas9-based genome editing technology that leverages the non-homologous end joining (NHEJ) pathway.
CAR T-Cell Therapy in Hematologic Malignancies: Mechanisms, Clinical Applications, and Next-Generation Strategies
This article provides a comprehensive analysis of the mechanism of action of chimeric antigen receptor (CAR) T-cell therapy for hematologic malignancies, tailored for researchers, scientists, and drug development professionals.
The Evolution of CAR-T Cell Therapy: From First-Generation Constructs to Fifth-Generation Designs
This article provides a comprehensive review of the groundbreaking evolution of Chimeric Antigen Receptor T-cell (CAR-T) therapy, tracing its development from foundational first-generation constructs to sophisticated fifth-generation designs.
Circuit Compression in Synthetic Biology: A Comparative Analysis of T-Pro vs. Inversion-Based Genetic Circuit Design
This article provides a comprehensive comparison of circuit compression techniques in synthetic biology, focusing on the emerging Transcriptional Programming (T-Pro) approach against traditional inversion-based methods.
Transcriptional vs. Post-Transcriptional Control: Navigating Gene Expression Burden in Biomedical Research
This article provides a comprehensive comparison of transcriptional and post-transcriptional control mechanisms, with a specific focus on understanding and mitigating the cellular burden associated with gene expression.
Strategies to Mitigate Cellular Burden in Synthetic Gene Circuits: From Foundational Concepts to Clinical Applications
Synthetic gene circuits often impose a significant metabolic burden on host cells, leading to reduced growth and the eventual dominance of non-functional mutants, which severely limits their long-term utility in...
Strategies for Extending the Evolutionary Longevity of Synthetic Gene Circuits
This article provides a comprehensive overview of the latest strategies to combat the evolutionary instability of synthetic gene circuits, a major roadblock in biomedical and biomanufacturing applications.
Retroactivity in Synthetic Gene Networks: From Foundational Principles to Clinical Applications
This article provides a comprehensive analysis of retroactivity, a critical form of context-dependence in synthetic gene networks where downstream modules adversely interfere with upstream components by sequestering or modifying signals.
Resource Competition for RNAP and Ribosomes: Navigating Bottlenecks in Synthetic Genetic Circuit Design
This article explores the critical challenge of resource competition for shared cellular machinery, specifically RNA polymerase (RNAP) and ribosomes, in the design and function of synthetic genetic circuits.
Strategies for Addressing Metabolic Burden in Biosensor Circuits: From Foundational Principles to Clinical Applications
This article provides a comprehensive examination of metabolic burden in biosensor circuits, a critical challenge facing researchers in synthetic biology and drug development.