Research Articles
Advanced Strategies for Tandem CRISPR sgRNA Array Construction: A Comprehensive Guide to Efficient Multiplexed Genome Editing
This article provides a comprehensive overview of modern approaches for constructing tandem CRISPR sgRNA arrays to achieve efficient multiplexed genome editing.
Cell-Free TXTL Systems: Accelerating Synthetic Biology and Drug Development through Rapid Pathway Prototyping
Cell-free transcription-translation (TXTL) systems have emerged as a transformative platform for rapid prototyping of biological pathways, bypassing the constraints of living cells.
Synthetic Promoters for Natural Product Discovery: Refactoring Gene Clusters to Unlock Novel Therapeutics
This article provides a comprehensive overview of the strategies and technologies for refactoring natural product biosynthetic gene clusters (BGCs) using synthetic promoters.
Combinatorial Biosynthesis of Novel Polyketides and Non-Ribosomal Peptides: Engineering Assembly Lines for Drug Discovery
This article provides a comprehensive overview of combinatorial biosynthesis, a powerful synthetic biology approach that re-engineers the enzymatic assembly lines of polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) to...
Precision Control of Metabolic Pathways: Optimizing Bioproduction with Promoter and RBS Libraries
This article explores the cutting-edge methodologies of metabolic pathway optimization through the strategic use of promoter and Ribosome Binding Site (RBS) libraries.
PS-Brick: The Iterative Seamless DNA Assembly Framework Accelerating Metabolic Engineering and Synthetic Biology
This article explores the PS-Brick DNA assembly method, a novel framework that combines Type IIP and IIS restriction enzymes for iterative, seamless, and repetitive sequence cloning.
A Complete Guide to Golden Gate Assembly for Complex Multigene Constructs
This article provides a comprehensive resource for researchers and drug development professionals on employing Golden Gate Assembly for constructing complex multigene systems.
Overcoming the Hurdles: A Comprehensive Guide to Challenges in Heterologous Expression of Biosynthetic Gene Clusters
Heterologous expression of biosynthetic gene clusters (BGCs) is a powerful strategy for discovering novel natural products with pharmaceutical potential, yet it is fraught with technical challenges that limit its success...
Yeast Homologous Recombination: A Comprehensive Guide to DNA Assembly for Biomedical Research
This article provides researchers, scientists, and drug development professionals with a thorough examination of yeast homologous recombination (YHR) for DNA assembly.
Unlocking Silent Code: Strategies for Activating Cryptic Bacterial Gene Clusters for Novel Natural Product Discovery
This article provides a comprehensive overview of cryptic or silent biosynthetic gene clusters (BGCs) in bacteria, which represent a vast untapped reservoir of novel natural products.