Research Articles
Synthetic Biology Circuits: Design Principles, Methodologies, and Clinical Translation for Drug Development
This article provides a comprehensive resource for researchers and drug development professionals on the fundamentals of synthetic gene circuits.
Precise Metabolic Engineering: A Comprehensive Guide to dCas9 sgRNA Design for Effective Pathway Knockdown
This article provides a complete framework for designing highly effective dCas9 sgRNAs tailored for metabolic pathway knockdown.
Microbial Consortia Engineering: Distributing Metabolic Pathways for Advanced Biomanufacturing and Therapeutics
This article explores the paradigm of microbial consortia engineering for distributing complex metabolic pathways across multiple specialized strains.
BioBrick vs BglBrick vs Golden Gate: A Comprehensive Comparative Analysis for Synthetic Biology and Drug Development
This article provides a detailed comparative analysis of three foundational DNA assembly standards in synthetic biology: BioBrick, BglBrick, and Golden Gate.
HDR vs NHEJ: Decoding DNA Repair Efficiency for Precision Genome Editing and Therapy
This article provides a comprehensive analysis of the efficiency and application of the two primary DNA double-strand break repair pathways: Homology-Directed Repair (HDR) and Non-Homologous End Joining (NHEJ).
Optimizing DNA Assembly: Strategies for High-Efficiency, High-Fidelity Large Constructs
The synthesis of large DNA constructs is a cornerstone of synthetic biology and therapeutic development, yet achieving high efficiency and fidelity remains a significant challenge.
Molecular Scissors and Glue: How Restriction Enzymes and DNA Ligase Power Modern Cloning
This article provides a comprehensive overview of the indispensable roles restriction enzymes and DNA ligase play in molecular cloning, a cornerstone technique for researchers, scientists, and drug development professionals.
CRISPR-Cas System Classification: From Foundational Types to Therapeutic Applications in Drug Development
This comprehensive review explores the evolving classification of CRISPR-Cas systems, from foundational principles to cutting-edge applications in biomedical research.
CRISPR vs TALEN vs ZFN: A 2025 Efficiency Comparison for Research and Therapeutic Applications
This article provides a comprehensive, up-to-date comparison of the three primary genome editing technologies—CRISPR, TALEN, and ZFN—tailored for researchers, scientists, and drug development professionals.
Minimizing Indel Mutations in Genome Editing: Strategies for Enhanced Fidelity in Therapeutic Development
This article provides a comprehensive analysis of insertion/deletion (indel) mutations, a significant byproduct of CRISPR-based genome editing that poses challenges for research and therapeutic applications.