Research Articles
A Practical Framework for Validating Synthetic Data in Computational Biology Benchmarks
The adoption of synthetic data in computational biology is accelerating, offering solutions to data scarcity, privacy constraints, and the need for robust benchmark studies.
CAR-T Cell Generations in Oncology: A Comparative Analysis of Structure, Efficacy, and Clinical Translation
This article provides a comprehensive comparative analysis of the five generations of Chimeric Antigen Receptor T-cell (CAR-T) therapies, from foundational concepts to cutting-edge innovations.
Benchmarking Differential Abundance Tests for Microbiome Data: A Realistic Guide for Robust Biomarker Discovery
Differential abundance (DA) analysis is a cornerstone of microbiome research, essential for identifying microbial biomarkers linked to health, disease, and environmental outcomes.
Synthetic Biology and Metabolic Engineering: Advanced Strategies to Boost Actinobacterial Natural Product Titers
Actinobacteria are prolific producers of bioactive natural products, yet their low production titers and silent biosynthetic gene clusters (BGCs) present major challenges for drug development and industrial application.
Circuit Failure in Biological Systems: From Molecular Pathophysiology to AI-Driven Drug Development
This article addresses the critical challenge of circuit failure in complex biological systems, a central problem in developing treatments for neuropsychiatric and neurological disorders.
CRISPRi for Fine-Tuning Metabolic Pathways: A Comprehensive Guide for Researchers
This article provides a detailed exploration of CRISPR interference (CRISPRi) as a powerful tool for the precise, multi-level regulation of metabolic pathways in both bacterial and eukaryotic systems.
Optimizing Gene Expression Using Orthogonal Regulators: From Foundational Concepts to Therapeutic Applications
Orthogonal gene expression systems represent a transformative approach in synthetic biology, enabling precise, independent control of multiple genetic elements without cross-talk with host regulatory networks.
Overcoming Metabolic Burden in Microbial Cell Factories: Strategies for Robust Bioproduction
This article provides a comprehensive overview of the challenges and solutions associated with metabolic burden in engineered microbial cell factories.
Combinatorial Optimization of Enzyme Expression Levels: From Foundational Concepts to Advanced Applications in Drug Development
This article provides a comprehensive overview of combinatorial optimization strategies for balancing enzyme expression levels in engineered metabolic pathways, a critical challenge in metabolic engineering and pharmaceutical development.
Strategic Optimization of Biomass and Product Yield in Metabolic Engineering: From Foundational Principles to Industrial Translation
This comprehensive review addresses the central challenge of optimizing biomass utilization and product yield in metabolic engineering, a critical pursuit for researchers and drug development professionals.