Research Articles
Circuit Failure in Biological Systems: From Molecular Pathophysiology to AI-Driven Drug Development
This article addresses the critical challenge of circuit failure in complex biological systems, a central problem in developing treatments for neuropsychiatric and neurological disorders.
CRISPRi for Fine-Tuning Metabolic Pathways: A Comprehensive Guide for Researchers
This article provides a detailed exploration of CRISPR interference (CRISPRi) as a powerful tool for the precise, multi-level regulation of metabolic pathways in both bacterial and eukaryotic systems.
Optimizing Gene Expression Using Orthogonal Regulators: From Foundational Concepts to Therapeutic Applications
Orthogonal gene expression systems represent a transformative approach in synthetic biology, enabling precise, independent control of multiple genetic elements without cross-talk with host regulatory networks.
Overcoming Metabolic Burden in Microbial Cell Factories: Strategies for Robust Bioproduction
This article provides a comprehensive overview of the challenges and solutions associated with metabolic burden in engineered microbial cell factories.
Combinatorial Optimization of Enzyme Expression Levels: From Foundational Concepts to Advanced Applications in Drug Development
This article provides a comprehensive overview of combinatorial optimization strategies for balancing enzyme expression levels in engineered metabolic pathways, a critical challenge in metabolic engineering and pharmaceutical development.
Strategic Optimization of Biomass and Product Yield in Metabolic Engineering: From Foundational Principles to Industrial Translation
This comprehensive review addresses the central challenge of optimizing biomass utilization and product yield in metabolic engineering, a critical pursuit for researchers and drug development professionals.
Solving the Puzzle: A Comprehensive Guide to Troubleshooting Host Context Problems in Heterologous Expression
Heterologous expression is a cornerstone of biotechnology for producing therapeutics, enzymes, and for natural product discovery.
Taming Contextual Effects: Strategies for Robust Synthetic Genetic Circuits in Biomedical Applications
Synthetic genetic circuits hold transformative potential for biotechnology and medicine, but their predictable design is hampered by pervasive contextual effects.
Microfluidics for Synthetic Biology: Advanced Tools for Biomanufacturing, Diagnostics, and Drug Development
This article explores the transformative integration of microfluidics and synthetic biology, a convergence driving breakthroughs in biomedical research and industrial bioprocesses.
Promoter Engineering for Rational Biosynthetic Gene Cluster Refactoring: Activating Silent Gene Clusters for Drug Discovery
This article provides a comprehensive overview of promoter engineering strategies for the rational refactoring of biosynthetic gene clusters (BGCs), a central challenge in natural product discovery.